The couple confirmed the news of their sad split to the Associated Press, sharing it was "mutual and lovingly made at the end of last year."
"Normally we would do this privately, but given that the gossip industry cannot resist an opportunity to speculate and invent, we wanted to convey the truth directly," Jennifer's publicist Stephen Huvane released in a statement.
"Whatever else is printed about us that is not directly from us, is someone else's fictional narrative. Above all, we are determined to maintain the deep respect and love that we have for one another."
What a time to be alive!
It had been noted that for the past few months both Jennifer and Justin had been spending time apart.
She recently celebrated her 49th birthday without her husband, surrounding herself with friends Courteney Cox and manager, Aleen Keshishian.
The last time they were seen together was in December 2017, where they holidayed in Mexico with pals Jason Bateman and Amanda Anka.
While their final official appearance as couple was at Jason's induction ceremony for a star on the Hollywood Walk of Fame, back in July 2017.
Justin's last Instagram post of Jen was in August 2017
Friends of the pair said that time apart really helped with their marriage.
"Justin often spends a few days in NYC by himself," an insider told People about the 46-year-old.
"When he is in NYC, Jen will catch up with friends and enjoys her own life. When Justin is in L.A., it's very special for them. They socialize together with friends. They often go out to dinner or have people over."
Just last February, Jen opened up her LA home to Architectural Digest.
Proudly showing off their love nest, she gushed, "I look around at my husband and my dogs and our home, and there's nowhere else I want to be."
Jen and Justin first met on the set of Tropic Thunder back in 2008.
After falling in love on the set of Wanderlust, the actors began dating in May 2011 and announced their engagement a year later in August.
Shocking the world, they tied the knot in a surprise ceremony in August 2015 - just days before Justin's 44th birthday.
Watch our exclusive chat with the stunning actress in the video below!
Friends, who were invited to the magical day under the ruse of Justin's birthday, were overjoyed for them.
"They seemed ecstatic," one attendee told People.
"They celebrated late and barely slept, but they were in the best mood. They were giddy to have pulled off an amazing wedding celebration with their friends. And they were proud to finally be married."
Last year, close sources to the couple spoke out about how their marriage was something they needed to work on constantly.
"It's possible that this marriage would not have worked as well if they were in their 20s, but they aren't. They are great friends and have total respect for each other," an insider revealed.
Was the distance too much?
Last year, Justin told TV WEEK that couple's work demands means they're not often in the same place for very long.
"It's tough, it's a pain," he said at the time.
"We have a two-week rule [to see each other]. But when you create those rules, they get blown out of the water pretty quickly."
"But when you are tired, you just make an extra effort to make trips back or have your partner come visit you. She [Jen] has an enormously good sense of humour," Justin gushed.
"We make each other laugh a lot – we really do. There's nothing that gives me more pleasure than watching her laugh hard and then getting her to laugh harder."
This was Justin Theroux's first wedding and Jennifer Aniston's second.
The actress was previously married from 2000-2005 to Brad Pitt, who called it quits with his second wife Angelina Jolie back in 2016.
While Jennifer herself is yet to talk about the demise of her second marriage, she has shared the pain of a break-up in the past.
In 2016, The actress showed her raw side during a panel at the 46th Annual Giffoni Film Festival.
"We're all human beings at the end of the day, whether we're a waitress or we're a baker or we're a student – whatever we are, at the end of the day, you can kind of hit walls and think 'I can't go any farther.' Or 'this is too much, my heart can't take it.' Or 'the pain is too great.' Or 'am I good enough?' Or, 'Will I survive?'"
Adding, "And you just have to sort of, somehow, miraculously overcome. You just go, 'I can.' Yes, you can!"
It really is the end of the era and we wish Jen and Justin all the best in this next chapter.